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. Author manuscript; available in PMC: 2010 Mar 28.
Published in final edited form as: Prostate. 2007 Apr 1;67(5):536–546. doi: 10.1002/pros.20549

Fig. 5.

Fig. 5

DC are essential for presenting prostatic antigen during tumorigenesis. Pro-HA single transgenic and Pro-HA × TRAMP double transgenic mice on the B10.D2 background were lethally irradiated and reconstituted with bone marrow from either control NT or CD11c-DTR (DTR) donors. At least 8 week slater, chimeras were treated with DT(4ηg/gbody weight)or PBS on Days −4, −1, and +2, and received adoptive transfers of naœve CFSE-labeled clonotypic CD4 cells on Day 0 which were subsequently recovered for analysis 5 days post-transfer from the prostate-draining LN. Chimeric recipients were between 3 and 4 months of age at the time of adoptive transfer. Representative CFSE-dilution histograms (A) and scatter plot showing the percentage of clonotypic CD4 cells with diluted CFSE for individual mice(B)are presented as in previous figures.