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. Author manuscript; available in PMC: 2010 Mar 28.
Published in final edited form as: Cancer Cell. 2005 Mar;7(3):239–249. doi: 10.1016/j.ccr.2005.01.027

Figure 4. Androgen ablation transiently increases presentation of prostate-epithelial antigen.

Figure 4

Pro-HA and Pro-HA × TRAMP mice castrated at the indicated time points received adoptive transfers of Thy1.1+ CFSE-labeled naive clonotypic CD4 cells, which were recovered from the prostate-draining periaortic LNs 6 days later.

A: Representative proliferative responses (i.e., CFSE dilutions) of clonotypic CD4 cells.

B: Scatter plot showing the effect of androgen ablation on clonotypic CD4 cell proliferation is presented as in Figure 3B.

C: Representative histogram plots of IFN-γ staining, with the percentage of cytokine-positive clonotypic CD4 cells, as well as the level of cytokine expression on these positive cells (expressed as mean fluorescence [MF]) shown above and below the reference bar, respectively.

D: Comparison of CFSE dilution in clonotypic CD4 cells recovered from intact and castrated NT, vacc-HA-infected NT (vacc-HA), and C3-HA recipients.