Table 1. In vivo LV injection: effect on lymph node dendritic cell subset numbers and enGFP expression.
| Dendritic cell subset* | Cohort† | Total dendritic cell number per lymph node‡ |
enGFP+ dendritic cell number per lymph node |
||
|---|---|---|---|---|---|
| n | Statistics | n | Statistics | ||
| Myeloid | [A] LV, ipsilateral | 2.3 × 103 ± 0.4 | A vs C, P = 0.008 | 11 ± 3 | A vs C, P = 0.01 |
| [B] LV, contralateral | 1.5 × 103 ± 0.6 | B vs C, P = 0.08 | 19 ± 3 | B vs C, P = 0.20 | |
| [C] control | 0.4 × 103 ± 0.1 | 1 ± 1 | |||
| Plasmacytoid | [A] LV, ipsilateral | 2.0 × 103 ± 0.6 | A vs C, P = 0.05 | 3 ± 1 | A vs C, P = 0.04 |
| [B] LV, contralateral | 1.2 × 103 ± 0.3 | B vs C, P = 0.20 | 4 ± 1 | B vs C, P = 0.02 | |
| [C] control | 0.6 × 103 ± 0.1 | 1 ± 1 | |||
| Lymphoid | [A] LV, ipsilateral | 8.3 × 102 ± 3.0 | A vs C, P = 0.10 | BDL | |
| [B] LV, contralateral | 6.2 × 102 ± 2.3 | B vs C, P = 0.20 | BDL | ||
| [C] control | 3.2 × 102 ± 0.4 | BDL | |||
On day 3 after footpad injection of LV-enGFP, one ipsilateral and one contralateral inguinal lymph node was harvested from each mouse and controls. Total dendritic cells (DCs) and enGFP+ dendritic cells per lymph node were enumerated by flow cytometry. The following panels were used to identify DC subsets: myeloid, CD11c+, CD11b-B220-; plasmacytoid, CD11c+, B220+, Gr-1+; and lymphoid, CD11c+, CD11b-, B220-, CD8+.
Fourteen mice were injected with LV-enGFP; lymph nodes in each group were pooled from n = 2-3 mice, thereby giving overall n =5. n = 5 controls were also evaluated. Cell events analyzed ranged from 5.1 × 105 to 8.7 × 105 ipsilaterally, from 1.6 × 105 to 6.2 × 105 contralaterally, and 1 × 105 in controls.
Absolute numbers of cells per lymph node were calculated from flow and absolute count data. Mean ± SE. P values were determined by two-sided Student's t test. BDL, below detection limit (no events recorded).