Skip to main content
. Author manuscript; available in PMC: 2010 Mar 28.
Published in final edited form as: J Liq Chromatogr Relat Technol. 2008 Jan 1;31(19):3012–3019. doi: 10.1080/10826070802424956

Fig. 2.

Fig. 2

HPLC analysis of the crude sample and elatine from HSCCC separation. Separation column: a C18 column (4.6 mm I.D. × 250 mm, 5 μm); column temperature: 35°C; detection wavelength: 235 nm; The mobile phase: a linear gradient of methanol (A) and 0.4% phosphoric acid (B) as follows: A–B (25:75, v/v) to A–B (35:65, v/v) in 15 min, then to A–B (40:60, v/v) in 5 min, then to A–B (40:60, v/v) in 10 min, then to A–B (60:40, v/v) in 5 min. The flow-rate was 1.0 ml min−1. (A) Crude sample; (B) elatine