Maternal high-fat diet consumption causes perturbations in the serotonergic system of fetal offspring. a–c, HFD offspring displayed increased TPH2 mRNA in the rostral raphe (a; p = 0.03, n = 8), increased 5-HT1AR mRNA (b; p = 0.03, n = 6); and no difference in SERT mRNA (c; p = 0.60, n = 5) compared to CTR offspring. Aq, Aqueduct; 4N, trochlear nerve; MnR, median raphe nucleus. Scale bars, 1 mm.