Maternal high-fat diet consumption increased anxiety-like behavior in female offspring. a, b, Female offspring from HFD-fed mothers display increased latency to touch Mr. Potato Head (a; p = 0.01) and the rubber snake (b; p = 0.04) compared to female CTR offspring. c, d, There was no difference in the latency to touch the hanging bird toy (c; p = 0.16) or the kiwi fruit (d; p = 0.32) between female HFD and CTR offspring. Male HFD and CTR offspring had a similar response to Mr. Potato Head (a; p = 0.41), the rubber snake (b; p = 0.75), the hanging bird toy (c; p = 0.66), and the kiwi fruit (d; p = 0.95). Data are presented such that each point represents an animal (●, male offspring; ×, female offspring. *p < 0.05 when comparing female CTR and HFD offspring. For the CTR group, n = 4 male and 4 female offspring; for the HFD group, n = 12 male and 11 female offspring.