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. Author manuscript; available in PMC: 2011 Feb 5.
Published in final edited form as: Cell Stem Cell. 2010 Feb 5;6(2):117–129. doi: 10.1016/j.stem.2009.12.015

Figure 1. The pool of quiescent Pax7 satellite cells returns to homeostasis during adult muscle regeneration.

Figure 1

(A) Transverse sections of uninjured (0 days after injury) and regenerating (4, 12, and 50 days after injury) TA muscle contain quiescent (MyoD-, Ki67-) (green arrowhead) and cycling (MyoD+, Ki67+; yellow arrowhead) Pax7+ cells in sublaminar location (laminin+; magenta). Differentiating myogenic cells (red arrowhead) could be observed in regenerating muscle underneath the basal lamina. (B) Total number of Pax7+ cells (left) and quiescent (Ki67-; MyoD-) Pax7+ cells (right) per muscle section from 4-6 mice per time point (mean ± sem; p<0.05). (C) BrdU+/Pax7+ cells in transverse sections from fully regenerated muscle from BrdU-treated mice. Top panel: Pax7 (red) and DAPI (blue) staining, Bottom panel: BrdU (green) and DAPI staining. Regenerated muscle fibers are distinguished with DAPI+ central nuclei (white arrowhead). The contour of muscle fiber is denoted by white line. Scale bar; 40 μm (A), 80 μm (C).