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. Author manuscript; available in PMC: 2010 Mar 28.
Published in final edited form as: Am J Physiol Lung Cell Mol Physiol. 2007 Jun 8;293(2):L417–L428. doi: 10.1152/ajplung.00489.2006

Fig. 1.

Fig. 1

PGE2 suppresses the expression of collagen and smooth muscle actin-α (α-SMA). A: quiescent human lung fibroblasts (IMR-90) were treated with TGF-β1 (2 ng/ml), or TGF-β1 (2 ng/ml) and PGE2 (10 nM), under serum-free conditions for indicated times, and cell lysates were prepared. Cell lysates were subjected to SDS-PAGE followed by immunoblotting with antibodies against collagen 1, α-SMA, and β-actin. B: IMR-90 cells were treated with TGF-β1 alone, PGE2 alone (10 nM), or TGF-β1 + PGE2, and cell lysates were analyzed as above after 24 h. Data are representative of 3 independent experiments.