Fig. 11.

PGE₂ inhibits the TGF-β1-stimulated Y-397 FAK autophosphorylation in adult lung fibroblasts. A: quiescent adult lung fibroblasts were treated with TGF-β1 (2 ng/ml), or TGF-β1 (2 ng/ml) and PGE₂ (100 nM), under serum-free conditions, and cell lysates were prepared at the times indicated. B: quiescent adult lung fibroblasts were treated with PGE₂ (100 nM) under serum-free conditions, and cell lysates were prepared at the times indicated. Cell lysates were subjected to SDS-PAGE followed by immunoblotting with antibodies against Y397pFAK. Blots probed with antibody against Y397pFAK were stripped and blotted with antibodies against total FAK sequentially. C: densitometry data for n = 3 experiments as in A. D: densitometry data for n = 3 experiments as in B. Significance is indicated with # and * symbols as in Fig 4.