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. Author manuscript; available in PMC: 2010 Mar 28.
Published in final edited form as: Am J Physiol Lung Cell Mol Physiol. 2007 Jun 8;293(2):L417–L428. doi: 10.1152/ajplung.00489.2006

Fig. 6.

Fig. 6

PGE2 inhibits the TGF-β1-stimulated Y-397 focal adhesion kinase (FAK) autophosphorylation (Y397pFAK). A: quiescent human lung fibroblasts (IMR-90) were treated with TGF-β1 (2 ng/ml), or TGF-β1 (2 ng/ml) and PGE2 (10 nM), under serum-free conditions, and cell lysates were prepared at the times indicated. B: quiescent human lung fibroblasts (IMR-90) were treated with PGE2 (10 nM) under serum-free conditions, and cell lysates were prepared at the times indicated. Cell lysates were subjected to SDS-PAGE followed by immunoblotting with antibodies against Y397pFAK. Blots probed with antibody against Y397pFAK were stripped and blotted with antibodies against FAK sequentially. C: densitometry data for n = 3 experiments as in A. D: densitometry data for n = 3 experiments as in B. Significance is indicated with # and * symbols as in Fig 4.