Fig. 7.

PGE₂ inhibits the TGF-β1-stimulated PKB/Akt phosphorylation. A: quiescent human lung fibroblasts (IMR-90) were treated with TGF-β1 (2 ng/ml), or TGF-β1 (2 ng/ml) and PGE₂ (10 nM), under serum-free conditions, and cell lysates were prepared at the times indicated. B: quiescent human lung fibroblasts (IMR-90) were treated with PGE₂ (10 nM) under serum-free conditions, and cell lysates were prepared at the times indicated. Cell lysates were subjected to SDS-PAGE followed by immunoblotting with antibodies against phosphorylated Akt (S-473p-Akt). Blots probed with antibody against S-473p-Akt were stripped and blotted with antibodies against Akt sequentially. C: densitometry data for n = 3 experiments as in A. D: densitometry data for n = 3 experiments as in B. Significance is indicated with # and * symbols as in Fig 4.