Table 1.
List of primary antibodies and experiments
| Marker | Experiment | Host species | Immunogen | Dilution | Source | References | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | ||||||
| NeuN | + | Mouse | Purified cell nuclei, clone A60 | 1:50 | Chemicon/Millipore, Temecula, CA (MAB 377) | Mullen et al. (1992) | |||||||||
| Calbindin | + | Mouse | Chicken gut calbindin-D28k | 1:200 | Swant, Bellinzona, Switzerland (clone CL-300) | Celio et al. (1990) | |||||||||
| Calretinin | + | Rabbit | Human recombinant protein | 1:300 | Swant (serum 7699/4) | Schwaller et al. (1993) | |||||||||
| Choline acetyltransferase | + | Goat | Human placental enzyme | 1:25–1:50 | Chemicon/Millipore (AB144P) | Li and Furness (1998) | |||||||||
| Parvalbumin | + | Mouse | Carp muscle protein | 1:250 | Swant (clone PA-235) | Celio et al. (1988) | |||||||||
| Tyrosine hydroxylase | + | + | Rabbit | Denatured enzyme from rat | 1:100 | Chemicon/Millipore (AB152) | Haycock (1987) | ||||||||
| Tryptophan hydroxylase | + | Sheep | Recombinant rabbit enzyme pheochromocytoma | 1:200 | Chemicon/Millipore (AB1541) | Kruger et al. (2002) | |||||||||
| VGLUT1 | + | Mouse | GST fusion protein containing amino acids 523–560 of rat VGLUT1 | 1:50 | Synaptic Systems, Göttingen, Germany (clone F123;135001) | Montana et al. (2004) | |||||||||
| VGLUT2 | + | Rabbit | GST fusion protein containing amino acids 510–582 of rat VGLUT2 | 1:250 | Synaptic Systems (135102) | Montana et al. (2004) | |||||||||
| VGLUT3 | + | + | + | + | + | + | + | + | + | + | Guinea pig | GST fusion protein containing amino acids 530–588 of rat VGLUT3 | 1:300 | Dr. R.H. Edwards, San Francisco, CA | Harkany et al. (2003) |
| VGAT | + | Rabbit | Synthetic peptide (amino acids 75–87 in rat) | 1:250 | Synaptic Systems (131002) | Härtig et al. (2007) | |||||||||