Figure 5. Impaired UPRmt signaling in animals with reduced ClpX activity.
(A) Representative fluorescent photomicrographs of hsp-60pr∷gfp transgenic worms with a temperature sensitive mutation (zc32) that activates the UPRmt in an otherwise wildtype or d2030.2(tm2183) background. Where indicated wildtype or d2030.2(tm2183) worms were raised on k07a3.3(RNAi) for 3 days.
(B) A corresponding immunoblot of GFP expressed by hsp-60pr∷gfp transgenic worms in whom mitochondrial unfolded protein stress was induced. The worms were treated as indicated in (A).