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. Author manuscript; available in PMC: 2010 Mar 29.
Published in final edited form as: Mol Microbiol. 2009 Nov 10;75(1):92–106. doi: 10.1111/j.1365-2958.2009.06959.x

Fig. 3.

Fig. 3

Binding of ESAT6 and CFP10 to type 1 and type 2 pneumocytes. (A) WI26 cells or (B) A549 cells were incubated with ESAT6 or CFP10 (10 or 20 μg ml–1) or buffer (in triplicates) followed by exposure to anti-His antibodies and FITC-conjugated secondary antibodies were analysed by FACS. Only ESAT6 showed binding to the two cell types. The per cent FITC-labelled cells obtained when cells were incubated with either protein minus per cent FITC labelled cells exposed to anti-His antibodies and FITC-conjugated secondary antibody from a representative experiment is shown. The binding of ESAT6 to type 1 pneumocytes was significantly higher than type 2 cells at 10 μg ml–1 (*P = 0.032) and similar at 20 μg ml–1.