Coimmunoprecipitation with anti-ASC, anti-NLRP1 and preimmune serum of cortical lysates obtained from sham animals (Sh) and traumatized animals at 4 h after TBI. Immunoprecipitates were blotted for ASC, NLRP1, caspase-1, caspase-11, XIAP, pannexin 1 and caspase-3 (control). Anti-NLRP1 and anti-ASC immunoprecipitated NLRP1, processed caspase-1, caspase-11, pannexin 1, and the pro- and cleaved forms of XIAP, thus indicating association of these proteins in a multiprotein complex. Preimmune serum did not immunoprecipitate inflammasome proteins and was used as control.