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. Author manuscript; available in PMC: 2010 Mar 29.
Published in final edited form as: J Cereb Blood Flow Metab. 2009 Apr 29;29(7):1251–1261. doi: 10.1038/jcbfm.2009.46

Figure 3. TBI induces association of NLRP1 inflammasome proteins, processing of caspase-1, and cleavage of XIAP.

Figure 3

Coimmunoprecipitation with anti-ASC, anti-NLRP1 and preimmune serum of cortical lysates obtained from sham animals (Sh) and traumatized animals at 4 h after TBI. Immunoprecipitates were blotted for ASC, NLRP1, caspase-1, caspase-11, XIAP, pannexin 1 and caspase-3 (control). Anti-NLRP1 and anti-ASC immunoprecipitated NLRP1, processed caspase-1, caspase-11, pannexin 1, and the pro- and cleaved forms of XIAP, thus indicating association of these proteins in a multiprotein complex. Preimmune serum did not immunoprecipitate inflammasome proteins and was used as control.