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. Author manuscript; available in PMC: 2011 Mar 8.
Published in final edited form as: Vaccine. 2010 Jan 5;28(11):2323–2329. doi: 10.1016/j.vaccine.2009.12.064

Figure 2. Humoral responses after vaccination.

Figure 2

Four to five week old female BALB/c mice (n=6) were immunized i.n. with active rCPAF, inactive rCPAF, rGST, or PBS (mock) plus IL-12 on day 0, and booster immunizations were given on days 14 and 28. Mice also were given IL-12 alone on days −1 and +1. Ten days after the final immunization, mice were bled, sera were obtained and analyzed for total antibody and various isotypes of antibodies against active rCPAF using an indirect ELISA. Results are represented as mean ± SE of reciprocal titers corresponding to the 50% maximum binding. Titers of rCPAF specific antibodies induced by immunization with active rCPAF immunization were significantly (P ≤ 0.05; Student’s t test) greater than those induced by immunization with inactive rCPAF or GST or PBS. Results are representative of two individual experiments.