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. Author manuscript; available in PMC: 2010 Mar 29.
Published in final edited form as: J Biol Chem. 2004 Dec 6;280(7):5430–5434. doi: 10.1074/jbc.M413513200

FIG. 4. Translocation of the soluble E-subunit to membranes is agonist-dependent.

FIG. 4

Acini were treated with medium as control (Ctl) or supraphysiologic concentrations of 0.1 µM caerulein (Cer), 1 mM carbachol (Carb) (A), or 10 µM thapsigargin (Thapsi) (B) for 15 min and then homogenized. A membrane fraction was formed from a post-nuclear supernatant, and the E subunit was detected using immunoblot analysis (see “Materials and Methods”). Results are representative of three separate studies.