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. Author manuscript; available in PMC: 2010 Mar 29.
Published in final edited form as: J Biol Chem. 2004 Dec 6;280(7):5430–5434. doi: 10.1074/jbc.M413513200

FIG. 6. Caerulein stimulation causes a time-dependent translocation of soluble V-ATPase subunits to membranes in pancreatic acini.

FIG. 6

Acini were treated with 100 nM caerulein for the indicated periods and fractionated to obtain cytosolic (A) and particulate (B) fractions. Proteins were separated by SDS-PAGE and immunoblotted with anti-E subunit antibodies. Representative immunoblots are shown (n = 3). Band intensities were quantified by laser densitometry and expressed as mean ± S.E. *, p < 0.02 versus control (Ctl) for panels A and B.