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. Author manuscript; available in PMC: 2010 Jul 21.
Published in final edited form as: Biochemistry. 2009 Jul 21;48(28):6674–6683. doi: 10.1021/bi900322a

Figure 1. PKC isoforms phosphorylate the α1c subunit.

Figure 1

A, Schematic is shown of the α1c subunit with the intracellular segments used to construct GST fusion proteins. B, α1c subunit-GST fusion proteins bound to glutathione-sepharose were subjected to PKC kinase reaction with [γ-32P] ATP. The amount of PKC isoform used for each kinase assay was normalized based upon moles of phosphate transferred to test substrate. The amount of GST fusion protein utilized is shown in the Coomassie-stained gel. The bands at ~ 80-100 kDa are autophosphorylated PKC. All blots are representative of 3 or more similar experiments.