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. Author manuscript; available in PMC: 2010 Jul 21.
Published in final edited form as: Biochemistry. 2009 Jul 21;48(28):6674–6683. doi: 10.1021/bi900322a

Figure 4. PKC isoforms phosphorylate α1c Ser1928.

Figure 4

A, GST fusion proteins (WT and S1928A 1906-2170 fragment) were phosphorylated by PKC isoforms and PKD, size- fractionated, transferred to nitrocellulose and immunoblotted with a phospho-specific antibody recognizing phosphorylated Ser1928 (pS1928). B, Extracts from HEK cells transfected with WT or S1928A were prepared, followed by α1c immunoprecipitation and kinase reaction by the different PKC isoforms and PKD. Samples were size-fractionated on SDS-polyacrylamide gel, transferred to nitrocellulose membrane and probed with anti-phospho-Ser1928 antibody (upper panel) or α1c antibody (lower panel).