Figure 7. Ser1674 and Ser1928 phosphorylation is altered in PKCα TG and KO mice.
Heart extracts were prepared from PKCα TG and KO mice and α1c or preimmune (PI) immunoprecipitates were size-fractionated on SDS-PAGE, transferred to nitrocellulose and blotted with (A-B) anti-pS1674 or (C-D) anti-pS1928 antibodies (upper panels) or anti-α1c antibody (middle panel). Bar graphs of densitometric quantification of relative pS1674 and pS1928 phosphorylation (normalized to α1c immunoprecipitation; littermate control normalized to 1; n=3, *, p<0.05).
