Figure 2.
Effects of unilateral injection of siGHRHR on sleep. Rats received 0.1 nmol of siGHRHR on one side of the Sctx and scrambled siRNA on the contralateral Sctx at dark onset. A, EEG delta wave (0.5–4 Hz) power on the siGHRHR-receiving side (●) was significantly lower than the control side (○) during 20–24 h after injection (*p < 0.05; n = 7; two-way ANOVA repeated measures followed by Fisher's LSD multiple-comparison test). There was no significant difference between two sides on day 2 after injection. B, NREMS EEG power change from the baseline on the siGHRHR-receiving side (●) and the scrambled siRNA-receiving side (○) during 20–22 h after dark onset injections of siGHRHR. The asterisks (**) indicate significant differences between two sides (n = 7; p < 0.01; two-way ANOVA repeated measures followed by Fisher's LSD multiple-comparison test). Error bars indicate SEM.
