Table 1.

Quantified selectivity values for protein kinases discussed in the text. Peptide array data were quantified and normalized to an average value of 1 within a position. Positively selected residues with values greater than 1.5 are shown. Complete quantified PWMs for all kinases are provided as Dataset S1.

	Position
Kinase	−5	−4	−3	−2	−1	+1	+2	+3	+4
Atg1		W (1.8)	L (2.8)	A (1.6)		V (2.4)	Y (2.8)	L (1.7)	D (1.8)
			M (2.4)			I (2.3)	I (2.3)
						F (1.9)	F (2.1)
						M (1.9)	M (1.8)
							W (1.6)
							V (1.6)
Gin4	L (2.0)	R (2.7)	R (9.9)	S (7.9)	M (2.9)	M (2.6)	W (2.4)	N (2.8)	L (3.9)
	I (1.8)	K (2.4)	K (1.6)	T (1.8)	K (1.8)		Y (2.2)	D (1.8)	I (2.3)
	M (1.7)	H (1.8)		R (1.6)	P (1.8)			F (1.6)
	R (1.6)
	K (1.6)
Mps1			D (1.9)	D (4.5)	D (1.6)	M (1.9)	H (1.6)
			R (1.9)	E (2.4)		V (1.7)
				R (2.2)		I (1.7)
Prk1	L (4.5)		R (4.0)	Q (3.4)	Y (2.2)	G (6.8)
	I (2.7)		P (1.9)	H (2.0)	H (1.9)
	M (1.6)			R (1.9)
Snf1	L (2.5)	R (2.8)	R (7.5)	R (3.0)	R (2.2)	M (1.9)		N (2.1)	L (4.5)
	I (2.0)	K (2.5)	K (3.0)	T (2.4)	K (1.8)	F (1.9)		F (1.7)	I (2.1)
		H (1.7)		S (2.2)	H (1.7)	C (1.8)			M (1.7)
				C (1.7)	Y (1.7)	Y (1.6)
				V (1.6)
Kin1	K (1.7)	H (2.0)	H (2.2)	N (4.0)	R (2.0)	Q (1.6)		D (2.2)	L (5.5)
		W (2.0)	W (2.0)	S (2.3)		R (1.6)		N (1.7)	I (2.4)
			F (1.9)					C (1.6)	V (1.9)
			Y (1.6)
Kss1		H (1.8)		P (6.2)	D (2.0)	P (8.5)		P (1.9)	K (1.7)
					A (1.9)
					R (1.9)
Yak1	R (1.7)	R (2.4)	R (8.0)	R (3.0)	R (2.5)	P (5.5)	C (1.8)	P (1.7)
				C (2.5)	K (2.0)	R (2.3)
				P (2.1)	H (1.6)
				A (1.6)
Vhs1	M (6.4)	R (2.1)	R (11)	S (3.1)	K (1.8)		S (2.7)		F (1.6)
		K (1.6)		T (2.7)	H (1.7)		T (2.0)
		V (1.6)		R (2.0)