Table 3.
Proteins phosphorylated by Prk1 in vitro. Proteins functionally associated with actin rearrangement or endocytosis are highlighted. Ub, ubiquitin; RhoGAP, Rho guanosine triphosphatase-activating protein; SCF, Skp1-Cullin-F-box; UPR, unfolded protein response; WASP, Wiscott-Aldrich Syndrome protein.
| ORF | Protein name | Function |
|---|---|---|
| YBL024W | Ncl1 | m5C-methyltransferase |
| YBL047C | Ede1 | Key endocytic protein; binds plasma membrane in a Ub-dependent manner |
| YCR030C | Syp1 | Overexpression suppresses a pfy1 (profilin) null mutation |
| YDL001W | Rmd1 | Cytoplasmic protein required for sporulation |
| YDR159W | Sac3 | Nuclear pore-associated protein; suppressor of actin mutations |
| YER155C | Bem2 | RhoGAP involved in the control of cytoskeleton and morphogenesis |
| YGR094W | Vas1 | Mitochondrial and cytoplasmic valyl-tRNA synthetase |
| YHL030W | Ecm29 | Major component of the proteasome |
| YHR161C | Yap1801 | Protein involved in clathrin cage assembly; binds clathrin and Pan1 |
| YHR098C | Sfb3 | Involved in sorting Pma1 into COPII vesicles |
| YJL090C | Dpb11 | Subunit of DNA polymerase II epsilon complex |
| YJL129C | Trk1 | Component of the Trk1-Trk2 potassium transport system |
| YJL184W | Gon7 | Component of the EKC/KEOPS complex |
| YJR137C | Ecm17 | Sulfite reductase beta subunit involved in amino acid biosynthesis |
| YML088W | Ufo1 | F-box protein subunit of the SCF E3 ubiquitin ligase complex |
| YML103C | Nup188 | Subunit of the nuclear pore complex |
| YNL243W | Sla2 | Transmembrane protein that links actin to clathrin and endocytosis |
| YNL287W | Sec21 | Gamma subunit of coatomer complex |
| YOR093C | Yor093c | Function unknown; deletion causes sensitivity to UPR-inducing agents |
| YOR181W | Las17 | Actin assembly factor; homolog of human WASP |
| YPL131W | Rpl5 | Component of the large 60S ribosomal subunit |
| YPR160W | Gph1 | Glycogen phosphorylase |