Figure 1. Identification of a new alternatively spliced murine CGI58.

(A) RT-PCR amplification of murine mRNAs from preadipoctes and adipocytes. (B) RT-PCR analysis of mouse adipose, liver and pancreas RNA. RT was performed with random priming and equivalent aliquots of cDNA were used for PCR amplification. The 1053 bp cDNA corresponds to the full length murine CGI-58(GeneBank accession number NM_026179); the 594bp cDNA was identified as a novel spliced CGI-58 variant. (C) A schematic picture of murine CGI-58 genomic structure and its putatived transcripts. Black boxes represents the 5′ and 3′ untranslated regions. (D) Murine CGI-58S cDNA sequence with coding sequences capitalized. Square Box shows the alternative spliced region encoded by exon 2 and exon 3. Underlined sequence is a putative lipid binding domain. The conserved GXNXG motif and HX₄D motif are indicated in bold in black and grey areas, respectively. Histidine (H329) and the putative third residue of the catalytic triad, either glutamate (E262) or aspartate (D303) are indicated by double underlines. (E) Transient expression of proteins in HeLa cells was analyzed by immunoblotting with anti-Myc antibody.