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. Author manuscript; available in PMC: 2010 Mar 29.
Published in final edited form as: J Leukoc Biol. 2007 May 31;82(3):613–618. doi: 10.1189/jlb.0506317

Fig. 6.

Fig. 6

CypA-mediated migration of activated CD4+ T cells does not require cell surface heparans. Groups of nonactivated and activated PBMCs were treated with Heparinase I prior to CD4+ T cell isolation. Each group was then tested for cyclophilin-mediated chemotaxis using 100 ng/ml CypA, with or without 10 μg/ml anti-CD147 mAb. (A) Bar graphs show the CypA-mediated chemotaxis of untreated versus heparinase-treated, nonactivated and activated CD4+ T cells. (B) Bar graphs show the CypA-mediated chemotaxis of activated CD4+ T cells and neutrophils, with and without heparinase treatment. (C) Same groups as A, with additional groups of heparinase-treated cells, which included anti-CD147 mAb during chemotaxis. All graphs show mean (±SE) chemotactic index for each groups, and n = 4 – 6 wells per group. Dashed lines delineate significant chemotaxis (>1.2). Studies were performed using cells from different donors.