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. Author manuscript; available in PMC: 2011 Mar 9.
Published in final edited form as: Curr Biol. 2010 Feb 18;20(5):446–451. doi: 10.1016/j.cub.2010.01.019

Figure 1. Spatial asymmetries of MAPK phosphorylation.

Figure 1

(A-C) Co-immunostaining of the anteroposterior (AP) gradient of phosphorylated MAPK, dpERK (green) and Bcd (Red). In all figures, embryos are oriented with anterior to the left and dorsal side up.

(D) An averaged AP gradients of dpERK at early cell cycle 14 obtained from 40 embryos. The dashed line indicates the reference level of dpERK. Quantification of the gradients reveals a clear AP asymmetry: The anterior levels are higher than the posterior levels.

(E) Histogram of the ratios of anterior to posterior peak levels of MAPK phosphorylation from 244 wild type embryos. Individual peak values of dpERK at the termini are determined as described in Suppl. Fig. 1. In wild type embryos, this ratio has mean of 1.87 and standard deviation of 0.64, which indicates that the anterior levels of dpERK are significantly higher than the posterior levels (t-test, p<10−5)