Table 2.
Peptide sequences, calculated log D, and their stability in rat serum described in this work. KP is palmitoylation of Nζ of lysine. Calculated log D was determined from HPLC retention times. Serum stability was determined by incubation of peptide in 25% rat serum at 37° C.
| Analogue | Structure | Calc. log D | Rat Serum Stability, t½ (hour) |
|---|---|---|---|
| Gal-B2 | (Sar)WTLNSAGYLLGPKK(Kp)K | 1.24 ± 0.02 | 9.3 |
| [Sar1Ala]Gal-B2 | AWTLNSAGYLLGPKK(Kp)K | 1.28 ± 0.01 | n.d. |
| [Sar1Gly]Gal-B2 | GWTLNSAGYLLGPKK(Kp)K | 1.30 ± 0.04 | > 10 |
| [N-Ac, des-Sar]Gal-B2 | (N-Ac-Trp)TLNSAGYLLGPKK(Kp)K | 1.28 ± 0.02 | n.d. |
| [N-Me, des-Sar]Gal-B2 | (N-Me-Trp)TLNSAGYLLGPKK(Kp)K | 1.29 ± 0.01 | 9.0 |
| Gal-B5 | WTLNSAGYLLGPKK(Kp)K | 1.22 ± 0.02 | 10.0 |