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. Author manuscript; available in PMC: 2011 Feb 16.
Published in final edited form as: Biochemistry. 2010 Feb 16;49(6):1331–1337. doi: 10.1021/bi901810u

Figure 1.

Figure 1

(A) Eribulin (E7389) and its parent compound halichondrin B. The location of tritiation in [3H]eribulin is indicated by an asterisk (*). (B) Binding of eribulin to soluble tubulin. (C) Enlargement of (B) showing the binding of eribulin to soluble tubulin with the curve determined by non-linear regression. [3H]Eribulin was incubated with soluble MAP-depleted bovine brain tubulin (2 μM) for 20 min at 30 °C, followed by centrifugation through a microspin gel filtration column. Data are means ± SEM of three to six experiments.