Skip to main content
. Author manuscript; available in PMC: 2010 Mar 29.
Published in final edited form as: J Immunol. 2009 Jan 1;182(1):466–476. doi: 10.4049/jimmunol.182.1.466

FIGURE 4.

FIGURE 4

CHAC1 protein is expressed in the cytosol, and protein localization is not changed following induction of the UPR. a, Western blot detecting the V5 epitope, cytosolic control GAPDH, and nuclear histone H3 in cells transfected with C (CHAC1-V5eGFP (55 kDa)) or G (control eGFP). Whole indicates whole-cell extract, Cyto, cytosolic extract; Nuc, nuclear extract; Pellet, pelleted membranes. b, Fluorescence images of eGFP (green) and DAPI (blue) in cells transfected with CHAC1-V5eGFP-expressing plasmid or eGFP control. c, eGFP and DAPI fluorescence of HEK cells transfected with CHAC1-V5eGFP and treated with ox-PAPC or the chemical UPR inducers DTT, thapsigargin, or tunicamycin.