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CHAC1 overexpression induces apoptosis. HEK cells untransfected (UNT co) or transfected with CHAC1-V5 (CHAC1), GFP (GFP co), or CHAC1-V5eGFP (CHAC1-GFP). a, Fluorescent staining of nuclei with DAPI (blue), apoptoic nuclei with TUNEL-rhodamine (red), and GFP (green). Examples of apoptotic nuclei are highlighted with white arrows. b, Quantification of TUNEL-positive nuclei. *, p < 0.01.
