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. 2009 Sep 4;19(4):713–723. doi: 10.1111/j.1750-3639.2009.00318.x

Figure 4.

Figure 4

A. High‐grade astrocytoma used in subsequent immunohistochemical assays (hematoxylin–eosin, original magnification ×400). B. Antiepidermal growth factor receptor (anti‐EGFR) wild‐type immunohistochemistry showing strong diffuse cytoplasmic immunoreactivity, a pattern that is frequently associated with EGFR genetic amplification (original magnification ×400). C. Anti‐EGFRvIII immunohistochemical reactivity exhibiting strong cytoplasmic localization. EGFRvIII immunoreactivity is most commonly encountered in tumors also exhibiting amplification of the EGFR locus (original magnification ×400). D. Antiphosphatase and tensin homolog (anti‐PTEN) immunoreactivity demonstrating 80% of tumor cells with cytoplasmic reactivity, a pattern associated with an intact PTEN status in the tumor (original magnification ×400). E. Anti‐phospho‐S6 immunohistochemistry revealing approximately 20% of tumor cells labeling, indicating this messenger is activated in this tumor (original magnification ×400). F. Anti‐phospho‐Akt immunohistochemistry demonstrating approximately 80% of tumor cells labeling, indicating this messenger is activated in this tumor (original magnification ×400). Figure reproduced with permission from reference (63).