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. 2010 Mar 7;11:17. doi: 10.1186/1471-2121-11-17

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Copyright ©2010 Pasula et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Neither glucose nor the glucose sensors influence the subcellular localization of Mth1. (A) Yeast cells of the indicated genotype expressing GFP-Mth1 were grown to mid-log phase in a selective medium containing 2% galactose. Aliquots were then transferred to 2% galactose medium (Gal) or 4% glucose medium (Glu) and incubated for 60 min. Subcellular localization of GFP-Mth1 was analyzed by fluorescence microscopy. Yeast cells used were: WT (BY4742), rgt2Δsnf3Δ (YM6370), grr1Δ (YM4783), and rgt1Δ (YM4509). (B) Localization of a nondegradable form of Mth1 (Mth1-I85S) in wild-type cells (BY4742). (C) GFP-Rgt1 localization as a positive control.