Table 1.
Immunisation protocol and time points for collection of blood and spleens.
| Host | Time-points | Maternal treatment | |||
|---|---|---|---|---|---|
| PT mothers | CpG mothers | Al(OH)3 mothers | Naive mothers | ||
| Mothers | Day 3 of pregnancy. | Blood samples (T0) | |||
| 10 μg OVA + 0.5 μg PT N = 10 |
10 μg OVA + 50 μg CpG N = 10 |
10 μg OVA + 2 mg Al(OH)3 N = 10 |
Not immunised N = 10 |
||
| Day 10 of pregnancy | 1 μg OVA | 1 μg OVA + 5 μg CpG |
1 μg OVA + 0.2 mg Al(OH)3 |
Not immunised | |
| Day 10 after delivery | Blood samples (T10) | ||||
| 3 weeks after delivery | Exsanguinated (TT): serum and spleens collected | ||||
| Offspring | 6 weeks | Blood samples (T0) Immunised: 10 μg OVA + 2 mg Al(OH)3 N = 40 offspring per maternal treatment group |
|||
| 8 weeks | 10 μg OVA | ||||
| 9 weeks | Exsanguinated (TT): serum and spleen cells were collected | ||||