Figure 5.

In vitro migration assays reveal a role for both RHOA and PKRCZ. Individual and combined inhibition of PKRCZ and RHOA pathways strongly inhibits the capability of all the clones to migrate through 8 μm pores. OD values determined by Crystal Violet staining of the subclones migrated through the membrane of the Transwell Assay, without and in the presence of the inhibitory peptides indicated. The values of OD are proportional to the number of cells present on the lower surface of the transwell (average values from three individual experiments).