Figure 4.

Cluster analysis separates systemic and local response to infection. Expression levels of DEG 24 h after E. coli infection were converted to log₂ fold changes relative to the mean of untreated control animals. The four samples labeled with i24h represent i24h-1 to 4 of infected udder quarters. c₁24h comprises the four samples c24h-1, 2, 4 and 5 derived from udder quarters of untreated controls. The four samples subscripted with n24h were isolated from udder quarters neighboring to the quarters i24h-1 to 4 and the four samples labeled with c₂24h are replicates of c₁24h. These technical replicates allow a common representation of the two separately performed analyses of i24h and n24h. Expression values were clustered using the SOTA algorithm with Pearson's correlation metric in the program MeV4.2. Individual probe sets are represented as lines, red coloration indicates up-regulation upon treatment, green down-regulation. Magenta represents the per sample average.