Figure 3. NFV induces an increase in the sub-G1 DNA-containing population.

The DNA content degradation profiles of drug-sensitive and NFV-resistant axenic amastigotes (i.e. usually maintained under a constant drug pressure of 12.5 µM), which were either left untreated or treated with NFV (10 µM), were assessed by flow cytometry after cell permeabilisation and PI staining. DNA fragmentation was quantified by measuring the cell population in the sub-G1 DNA region indicated by M1 and G1 DNA peak indicated by M2. The data shown are representative of three independent experiments.