Figure 5. Loss of mitochondrial potential and translocation of EndoG in NFV-treated L. donovani amastigotes.

(A) L. donovani axenic amastigotes were exposed to the diluent or NFV (40 µM) for the indicated time periods. Mitochondrial membrane potential (Δψ_m) was assessed through the use of the JC-1 Mitochondrial Membrane Potential Detection Kit. Data are expressed as mean ± SD of three independent experiments. (B) Western blot analysis of the mitochondrial and cytosolic fractions obtained from NFV-treated amastigotes at different intervals. Anti-EndoG immunoblots of cytosolic and mitochondrial fractions are shown, along with the loading controls for mitochondria (i.e. COX IV) and cytosol (i.e. DHFR-TS), respectively.