Figure 6. Cytosolic translocation of EndoG in NFV-treated L. donovani amastigotes is inhibited by addition of antioxidant.

(A) L. donovani axenic amastigotes were exposed to NFV (40 µM) for 0 or 8 h with or without pre-treatment with NAC (20 mM). Western blot analysis of the cytosolic fractions obtained from the amastigotes was then performed using anti-EndoG (upper panel) and DHFR-TS (lower panel, as loading control). Densitometric analysis of blots from three of these experiments are shown in (B).