Table 2.
Effect of NeemAzal® on P. berghei oocyst prevalence and density on mosquito midguts.
| Treatment* | Experiment† | % Prevalence (CI95) | Oocysts/mosquito (CI95)# | Examined mosquitoes |
|---|---|---|---|---|
| control | 1 | 90 (81 - 99) | 17.5 (11.1 - 27.2) | 40 |
| 2 | 83 (68 - 99) | 29.9 (15.8 - 55.8) | 24 | |
| 3 | 100 | 111.2 (83.8 - 148.9) | 40 | |
| 4 | 86 (72 - 100) | 35.2 (21.6 - 57.6) | 22 | |
| 5 | 88 (78 - 98) | 11.3 (7.2 - 17.4) | 42 | |
| 6 | 89 (80 - 97) | 26.1 (15.8 - 42.4) | 53 | |
| tot‡ | 90 (86 - 94) | 27.7 (22.3 - 34.2) | 221 | |
| NA 13.2 mg/kg | 4 | 82 (64 - 100) | 49.9 (24.5 - 100.5) | 17 |
| 5 | 79 (66 - 91) | 12.7 (8.6 - 18.7) | 42 | |
| 6 | 100 | 39.4 (30.5 - 50.9) | 58 | |
| tot | 90 (84 - 95) | 28.9 (23.0 - 36.3) | 117 | |
| NA 25 mg/kg | 3 | 90 (81 - 99) | 42.8 (26.7 - 69.1) | 40 |
| 5 | 69 (55 - 84) | 8.9 (5.4 - 14.2) | 39 | |
| 6 | 96 (91 - 100) | 30.5 (22.3 - 41.1) | 54 | |
| tot | 86 (81 - 92) | 25.6 (19.9 - 32.8) | 133 | |
| NA 50 mg/kg | 1 | 0 | 0 | 55 |
| 2 | 0 | 0 | 43 | |
| 3 | 0 | 0 | 40 | |
| tot | 0 | 0 | 138 | |
| AZA 50 mg/kg | 3 | 95 (88 - 100) | 23.3 (15.0 - 36.0) | 41 |
| 5 | 88 (78 - 98) | 24.3 (17.2 - 34.5) | 42 | |
| 6 | 83 (68 - 99) | 3.2 (2.0 - 5.0) | 24 | |
| tot | 90 (73 - 100) | 16.1 (12.2 - 21.2) | 107 | |
Oocyst prevalence and intensity was assessed on day 10 after the infective blood meal carried out on mice treated with the indicated products and doses. * NA = NeemAzal® technical grade (Trifolio-M GmbH); AZA = azadirachtin tech grade (Sigma). † each number corresponds to one experimentation involving different treatment groups. # geometric means, only positive mosquitoes included. ‡ values refer to pooled data.