Figure 7.

Chronic exposure to 50 nM MMA(III) induces the sustained c-Jun translocation into the nucleus in UROtsa cells. Chronically exposed UROtsa cells were cultured in a 96 well plate in a density of 5 × 10³ cells/well. After 24 hr the cells were serum starved for additional 24 h, then fixed and permeabilized. Specific antibodies against phospho c-Jun were added to the plate. The secondary antibody labeled with Daylight-488 leads the cells nuclei to fluoresce in green-yellow when reached the target. Daylight-549 antibodies against total NFКβ were used for contrast in color images. The images were obtained using a deconvolution inverted microscope.