Table 1.
Escherichia coli K-12 strains and plasmids used in this study.
| Plasmid/Strain | Relevant genotype | Source or reference |
|---|---|---|
| pCP20 | FLP recombinase vector, temperature-sensitive origin, AmpR, CamR | [49] |
| pML104-3 | red+ gam+ under IPTG promoter, temperature-sensitive origin, SpecR, RecA+ | S. Elledge |
| BW26355 | ΔrecA635::FRTKanFRT | CGSC7651a; [49] |
| JW0525 | ΔintD::FRTKanFRT | [50] |
| JW1852 | ΔruvC::FRTKanFRT | [50] |
| JW1993 | ΔxonA::FRTKanFRT | [50] |
| JW2597 | ΔrecN::FRTKanFRT | [50] |
| JW3627 | ΔrecG::FRTKanFRT | [50] |
| JW4128 | ΔmutL::FRTKanFRT | [50] |
| MG1655 | sequenced wild-type E. coli K-12 | [51] |
| N2731 | recG258::Tn10miniKan | [44] |
| RDK1541 | recO1504::Tn5 | [52] |
| RTC0013 | ΔrecB::Kan | [53] |
| RTC0016 | ΔrecG::Kan | [53] |
| SMR6319 | 594 hsdrK− mK+ | [23] |
| SMR8976 | SMR6319 ΔuvrD404::FRTcatFRT | [23] |
| SMR8977 | SMR6319 ΔuvrA402::Gen | [23] |
| SMR8982 | SMR6319 ΔmutL482::FRT | [23] |
| SMR8986 | SMR6319 ΔmutL482::FRT ΔuvrA402::Gen | [23] |
| SMR8987 | ΔrecF1904::FRTcatFRT | [23] |
| SMR8991 | SMR6319 recA200(Ts) | [23] |
| SMR9801 | SMR6319 lexA3(Ind−) | [23] |
| SMR9805 | SMR6319 recAo281 lexA3(Ind−) | [23] |
| SMR9809 | SMR6319 recAo281 | [23] |
| SMR9811 | SMR6319 ΔuvrD404::FRTcatFRT metE163::Tn10 | [23] |
| SMR9812 | SMR6319 ΔuvrD404::FRTcatFRT ΔrecQ1906::FRT metE163::Tn10 | [23] |
| SMR9837 | SMR6319 sulA211 | [23] |
| SMR9847 | SMR6319 ΔrecJ::FRTKanFRT | [23] |
| SMR9932 | MG1655 recG258::Tn10miniKan | MG1655 x P1(N2731) |
| SMR10394 | SMR6319 ΔxonA::FRTKanFRT | SMR6319 x P1(JW1993) |
| SMR10395 | SMR6319 ΔxonA::FRT | SMR10394 x pCP20 |
| SMR10401 | SMR6319 ΔrecG::Kan | SMR6319 x P1(RTC0016) |
| SMR10407 | SMR6319 ΔruvC::FRTKanFRT | SMR6319 x P1(JW1852) |
| SMR10408 | SMR6319 ΔruvC::FRT | SMR10407 x pCP20 |
| SMR10411 | SMR6319 ΔrecF1904::FRTcatFRT | SMR6319 x P1(SMR8987) |
| SMR10416 | SMR6319 ΔruvC::FRT ΔxonA::FRTKanFRT | SMR10408 x P1(JW1993) |
| SMR10417 | SMR6319 ΔruvC::FRT ΔxonA::FRT | SMR10416 x pCP20 |
| SMR10419 | SMR6319 recG258::Tn10miniKan | SMR6319 x P1(SMR9932) |
| SMR10423 | SMR6319 ΔrecF::FRT | SMR10411 x pCP20 |
| SMR10424 | SMR6319 [pML104-3] | SMR6319 x pML104-3 |
| SMR10425 | SMR6319 ΔrecA635::FRTKanFRT | SMR6319 x P1(BW26355) |
| SMR10426 | SMR6319 ΔrecA::FRT | SMR10425 x pCP20 |
| SMR10427 | SMR6319 ΔrecA::FRT [pML104-3] | SMR10426 x pML104-3 |
| SMR10428 | SMR6319 ΔrecA::FRT ΔrecG::Kan [pML104-3] | SMR10427 x P1(RTC0016) |
| SMR10434 | SMR6319 ΔrecJ::FRT | SMR9847 x pCP20 |
| SMR10437 | SMR6319 ΔrecF::FRT recG258::Tn10miniKan | SMR10423 x P1(SMR9932) |
| SMR10438 | SMR6319 ΔxonA::FRT recG258::Tn10miniKan | SMR10395 x P1(SMR9932) |
| SMR10439 | SMR6319 ΔrecJ::FRT recG258::Tn10miniKan | SMR10434 x P1(SMR9932) |
| SMR10440 | SMR6319 lexA3(Ind−) recG258::Tn10miniKan | SMR9801 x P1(SMR9932) |
| SMR10441 | SMR6319 recAo281 lexA3(Ind−) recG258::Tn10miniKan | SMR9805 x P1(SMR9932) |
| SMR10442 | SMR6319 recAo281 recG258::Tn10miniKan | SMR9809 x P1(SMR9932) |
| SMR10443 | SMR6319 sulA211 recG258::Tn10miniKan | SMR9837 x P1(SMR9932) |
| SMR10678 | ruvA60 rus-1 ΔintD::FRTKanFRT | TNM759 x P1(JW0525) |
| SMR10698 | SMR6319 ΔrecG::Kan [pML104-3] | SMR10401 x pML104-3 |
| SMR10729 | SMR6319 ΔrecN::FRTKanFRT | SMR6319 x P1(JW2597) |
| SMR10730 | SMR6319 ΔrecN::FRT | SMR10729 x pCP20 |
| SMR10734 | SMR6319 ΔrecN::FRT recG258::Tn10miniKan | SMR10730 x P1(SMR9932) |
| SMR10735 | SMR6319 ΔrecF::FRT recG258::Tn10miniKan ΔuvrD::FRTcatFRT | SMR10437 x P1(SMR9811) |
| SMR10736 | SMR6319 ΔxonA::FRT recG258::Tn10miniKan ΔuvrD::FRTcatFRT | SMR10438 x P1(SMR9811) |
| SMR10737 | SMR6319 ΔrecJ::FRT recG258::Tn10miniKan ΔuvrD::FRTcatFRT | SMR10439 x P1(SMR9811) |
| SMR10738 | SMR6319 recG258::Tn10miniKan ΔuvrD::FRTcatFRT ΔrecQ::FRT metE163::Tn10 | SMR10419 x P1(SMR9812) |
| SMR10739 | SMR6319 recA200(Ts) recG258::Tn10miniKan | SMR8991 x P1(SMR9932) |
| SMR10740 | SMR6319 recA200(Ts) recG258::Tn10miniKan ΔuvrD::FRTcatFRT | SMR10739 x P1(SMR9811) |
| SMR10741 | SMR6319 ΔintD::FRTKanFRT | SMR6319 x P1(JW0525) |
| SMR10742 | SMR6319 ΔintD::FRTKanFRT rus-1 | SMR6319 x P1(SMR10678) |
| SMR10743 | SMR6319 ΔintD::FRT | SMR10741 x pCP20 |
| SMR10744 | SMR6319 ΔintD::FRT rus-1 | SMR10742 x pCP20 |
| SMR10745 | SMR6319 ΔintD::FRT recG258::Tn10miniKan | SMR10743 x P1(SMR9932) |
| SMR10746 | SMR6319 ΔintD::FRT rus-1 recG258::Tn10miniKan | SMR10744 x P1(SMR9932) |
| SMR11115 | SMR6319 ΔuvrA402::Gen recG258::Tn10miniKan | SMR8977 x P1(SMR9932) |
| SMR11116 | SMR6319 ΔmutL482::FRT recG258::Tn10miniKan | SMR8982 x P1(SMR9932) |
| SMR11117 | SMR6319 ΔmutL482::FRT ΔuvrA402::Gen recG258::Tn10miniKan | SMR8986 x P1(SMR9932) |
| SMR11132 | SMR6319 ΔrecG::FRTKanFRT | SMR6319 x P1(JW3627) |
| SMR11133 | SMR6319 ΔrecG::FRT | SMR11132 x pCP20 |
| SMR11134 | SMR6319 recO1504::Tn5 | SMR6319 x P1(RDK1541) |
| SMR11135 | SMR6319 ΔrecG::FRT recO1504::Tn5 | SMR11133 x P1(RDK1541) |
| SMR11186 | SMR6319 ΔrecB::Kan | SMR6319 x P1(RTC0013) |
| SMR11187 | SMR6319 ΔrecG::FRT ΔrecB::Kan | SMR11133 x P1(RTC0013) |
| SMR11188 | SMR6319 ΔrecG::FRT [pML104-3] | SMR11133 x pML104-3 |
| SMR11189 | SMR6319 ΔrecB::Kan [pML104-3] | SMR11186 x pML104-3 |
| SMR11190 | SMR6319 ΔrecG::FRT ΔrecB::Kan [pML104-3] | SMR11187 x pML104-3 |
| SMR11301 | SMR6319 lexA3(Ind−) ΔmutL::FRTKanFRT | SMR9801 x P1(JW4128) |
| SMR11303 | SMR6319 recAo281 lexA3(Ind−) ΔmutL::FRTKanFRT | SMR9805 x P1(JW4128) |
| SMR11305 | SMR6319 recAo281 ΔmutL::FRTKanFRT | SMR9809 x P1(JW4128) |
| SMR11318 | SMR6319 lexA3(Ind−) ΔmutL::FRT | SMR11301 x pCP20 |
| SMR11319 | SMR6319 recAo281 lexA3(Ind−) ΔmutL::FRT | SMR11303 x pCP20 |
| SMR11320 | SMR6319 recAo281 ΔmutL::FRT | SMR11305 x pCP20 |
| SMR11323 | SMR6319 ΔmutL::FRTKanFRT | SMR6319 x P1(JW4128) |
| SMR11325 | SMR6319 lexA3(Ind−) ΔmutL::FRT recG258::Tn10miniKan | SMR11318 x P1(SMR9932) |
| SMR11327 | SMR6319 recAo281 lexA3(Ind−) ΔmutL::FRT recG258::Tn10miniKan | SMR11319 x P1(SMR9932) |
| SMR11329 | SMR6319 recAo281 ΔmutL::FRT recG258::Tn10miniKan | SMR11320 x P1(SMR9932) |
| TNM759 | rus-1 ruvA60 | [39] |
a
CGSC – The Coli Genetic Stock Center, Yale