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. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: Dev Dyn. 2010 Apr;239(4):1102–1112. doi: 10.1002/dvdy.22259

Figure 7. In the absence of Sox9 function otic precursors showed reduced proliferation.

Figure 7

(A) Whole-mount phosphohistone H3 (PHH3) staining at stage 15. Lateral views, dorsal to top, anterior is to the right (control side) or to the left (injected side). The circled areas (red) outline the approximate position of the prospective otic epithelium in which PHH3-positive cells were counted. The scale bar represents 500 μm. (B) Higher magnification views of the circled areas of the embryos injected with 2.5 ng Sox9MO shown in panel (A). (C) Graph illustrating the quantification of PHH3-positive cells on control and injected sides in embryos that received unilateral injection of Sox9MO (1 ng, n=16; and 2.5 ng, n=15). (*) P-value is <0.0008; (**) P-value is <0.0003.