Figure 1.

Procedure for retrieval of pseudopodia from Boyden chamber filters. a. A Boyden chamber with four large wells was assembled with a gelatin coated filter (containing 3 or 1 µm pores) separating top and bottom wells. Chemoattractants were present in the bottom wells. Single cell suspensions were added to the top wells. b. After a 6 h incubation in cell culture conditions, the filter was removed and submerged in 100% methanol for 30 s. The filter was placed on a glass slide with unmigrated cell materials on the top surface. This material was gently wiped away completely with minimal pressure using large Kimwipes. Fresh Kimwipes were then used to apply firm pressure for 30 s to force adherence of pseudopodia to the glass. c. and d. The filter was then carefully peeled off while leaving behind pseudopodia adherent to the glass.