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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: J Hepatol. 2010 Feb 13;52(4):586–593. doi: 10.1016/j.jhep.2010.01.003

Figure 1. PO does not attenuate PA- mediated steatosis.

Figure 1

Nile red staining was performed on Huh-7 cells treated with vehicle (Veh), 200 µM PA, 200 µM PO plus 200 µM PA, or 200 µM PO alone for 18 h. (A) Representative fluorescent photomicrographs (× 60) are depicted. Nile red fluoresces as a yellow-gold at about 510nm; cells were counter-stained for nucleic acids by DAPI (blue). (B), Cellular steatosis was quantified in 5 random low power fields for each condition with image analysis software. Total area of lipid per cell (pixels above threshold) is represented. The data represent the mean ± SEM for n = 3 studies. (C) Huh-7 cells were treated with vehicle (Veh), 800 µM PA, 400 µM PO plus 800 µM PA, or 400 µM PO alone for 8 hours. SCD-1 mRNA was quantified by real time PCR. Fold induction was determined by normalization to 18S ribosomal RNA. Data represent the mean ± SEM of 3 independent experiments.