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. Author manuscript; available in PMC: 2011 May 15.
Published in final edited form as: Int J Cancer. 2010 May 15;126(10):2319–2329. doi: 10.1002/ijc.24920

Figure 3.

Figure 3

CXCL13 stimulates MMP-9 expression in OSCC cells. (A) Gelatin zymogram analysis of MMP-9 activity in CM obtained from OSCC cells (SCC14a, SCC1 and SCC12) were stimulated with and without CXCL13 (10 ng/ml) for 48 h. (B) OSCC cells were stimulated with and without CXCL13 (0–25 ng/ml) for 48 h. Total RNA isolated from these cells was analyzed for MMP-9 mRNA expression by real-time RT-PCR. Relative levels of MMP-9 mRNA expression was normalized with respect to the level of GAPDH amplification (*p<0.05). (C) OSCC cells were stimulated with CXCL13 (0–25 ng/ml) for 48 h and total cell lysates were analyzed by Western blot for MMP-9 expression. (D) Anti-CXCR5 antibody inhibits CXCL13-induced MMP-9 expression in OSCC cells. Rabbit non-specific IgG treatment severed as control.