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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Gastroenterology. 2009 Oct 22;138(4):1479–1490. doi: 10.1053/j.gastro.2009.10.034

Figure 6.

Figure 6

CARTp activates CREB and EGR1 to stimulate CART and Y2R expression. (A) Strong nuclear staining of phosphoCREB in vagal afferent neurons (arrows) transferred to serum-free medium (2 h, control) and stimulated with CARTp (2 nM). (B) Co-transfection with A-CREB (1 µg DNA per well) inhibited CARTp (2 nM, 16 h) stimulation of 3.45 kb CART-Luc, and CARTp only weakly stimulated expression of a Cre-deletion mutant of CART-Luc (ΔCre-CART-Luc). (C) CARTp (2 nM, 2 h) stimulated nuclear translocation of EGR1. (D) Mutation of a putative EGR1 binding site in CART-Luc (ΔEGR1-CART-Luc) reduced responses to CARTp and so too did pretreatment with EGR1 siRNA. (E) CARTp (2 nM, 16 h) stimulated expression of 3.034 bp Y2R-Luc and co-transfection with A-CREB inhibited this. (F) Pretreatment with EGR1 siRNA reduced CARTp stimulation of Y2R-Luc. Mean ± SE, n=6 independent experiments; ** p<0.01, *** p<0.001.