Fig. 1.

Mirk is a new regulator of osteosarcoma cell survival and apoptosis. (A) Results of lentiviral shRNA directed against Mirk in KHOS cells from the initial screen. The data are representative of a single well of a 96-well plate of MISSION® LentiExpress™ Human Kinases shRNA library as described in the Materials and Methods. (B) Mirk cDNA rescue assay. Pretransfection of full length of Mirk cDNA constructs that lack the 3′UTR could reverse the cell death phenotype when induced by a specific Mirk lentiviral shRNA, which targets the 3′UTR region. (C) Mirk expression in cDNA rescue assay as determined by western blot.