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. 2009 Dec 30;31(4):552–558. doi: 10.1093/carcin/bgp330

Fig. 3.

Fig. 3.

Knocked down Mirk expression decreases cell proliferation in multiple osteosarcoma cell lines. (A) Cell proliferation was determined by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-di-phenyltetrazolium bromide on cell lines: U-2OS, Saos, OSA344 and normal osteoblast cell HOB-c, after transduction with Mirk shRNA (NM_004714.x-442s1c1). The absorbance values were normalized by the control cells (non-infected) to 100% survival and the values of Mirk shRNA transduced cells were then quantified to get relative percentage of cell survival. Mirk knockdown decreases cell proliferation in osteosarcoma cells but not in normal osteoblast cells. (B) Phase contrast photomicrographs of cell lines before and after infection of lentiviral Mirk shRNA. Mirk knockdown induces significantly cell death in osteosarcoma cell lines U-2OS, Saos and OSA344 but not in normal osteoblast cell HOB-c.