Fig. 4.
Transcription activation-Gal4 reporter assay using full-length clones (1–754) for the wild-type (filled square) and A335V (filled diamond) mutation at different protein concentrations. The highest activation at 5 µg was found for a Gal-(1–715) clone (filled triangle, left side top). The empty vector pGLMRG5 was used as control (filled circle, left side down)